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Pada saat pemanasan media diharapkan tidak menggunakan suhu yang terlalu tinggi. Untuk alasan kepraktisan biasanya pembuatan media langsung dengan sekala besar dalam satu kali sterilisasi agar saat dibutuhkan stok media yang ada tinggal dipanaskan kembali.Weigh and dissolve the ingredients:
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Following completion of incubation, report the small print of observation in Report and work out The proportion of recovery for new lot with Look at to Earlier accredited media. If any new media obtaining for the first time, then inoculated microorganism shall be taken for calculation.Trypto